Establishment of Efficient Regeneration System of Populus×euramericana cl.‘Bofeng’

Compared with the species of Leuce section,the difficulty in plant regeneration during tissue culture for the species of Aigeiros section is a key limiting factor for their application in genetic engineering of forest trees.In this study,the plant growth regulators combination,copper concentration,light intensity,and the selection pressures of kanamycin were investigated using leaves of Populus×euramericana cl.’Bofeng’ as explants,and a stable and high efficient regeneration system was established.Using this system,both the shoot regeneration rate and rooting rate were up to 100%,and the average number of differentiated shoots in each leaf explant was up to 20,and the survival rate of seedlings reached 98%.The optimal selection pressure of kanamycin in shoot inducement for leaf-explant was 40 mg·L^-1,and the optimal selection pressure of kanamycin in root inducement of adventitious bud was 20 mg·L^-1.The authors also found that differentiation rate of adventitious buds of P.×euramericana cl.’Bofeng’ could be significantly promoted by increased copper concentration,and this is the first report about the key role of copper（Cu） in explant regeneration of Poplars.This regeneration system with high frequency which was established by the authors was as good as that of species of Leuce section,and it provides a good technological platform for genetic engineering of forest tree which uses the species of Aigeiros section as model materials.     

雄蚕品种秋华×平30在山西省的饲养试验

2012年秋季进行了雄蚕品种秋华×平30的饲养试验,结果表明:该品种蚕期发育齐一、眠起齐、抗性强,具有茧层厚、出丝率高、丝质优的特点。     

The Studies on the Grunting of Sows during Nursing and the Sucking Behaviour of Piglets

Two experiments were carried out to study the grunting of sows during nursing and the sucking be-haviour of piglets. In experimental I,six Yorkshire sows and their litters were observed and managed in farrowing crates.In experimental Ⅱ,six Harbin White sows and their litters were selected and housed in farrowing pens.The behaviours and activities of the sows during nursing and the actities of piglest during sucking were recorded with a portable video camera (PanasonicNV-VX22EN)The results showed that the characteristic patterns of grunting by the sows consist of an initial period of rhythmic grunting at a fairly constant rate and followesd by a period of more rapid grunting,and then a decline in grunting rate toward the completion of nursing.The pattern of vocalization showed by the mothers bears a reliable synchronization to the sucking behaviour of the piglets.The rate of grunting varied little during jostling for teat and nosing the udder.The beginning of slow sucking usually occurred during of just after the increase in grunt rate.The rapid sucking began 15-25seconds after the period of most rapid grunting,fol-lowingthe decrease of grunt rate.afterwards the nursing ended.There were individualities found in sows‘grunting patterns.It is found that vocalizations attracted the piglets to the sows and synchronized sucking activities of piglets,but the results indicated that the premassaging time and numbers of piglets enagaing massaging influ-enced the grunting pattern and the onset of the milk ejection.As more piglets were involved the massaging,the higher the grunting rate peak,the faster the milk ejection.     

杂交鳢(乌鳢♂×斑鳢♀)及其亲本血液指标的比较分析

本研究采用血细胞分析仪、流式细胞仪和血涂片制作方法,对杂交鳢及其亲本血液指标进行了比较分析。结果显示,杂交鳢(Channa argus♂×C.maculata♀)与其亲本相比,表现出明显杂种优势:在血液红细胞数目、白细胞数目、血红蛋白含量和血细胞比容上,杂交鳢都比亲本高,差异显著(P<0.05);在红细胞体积上,杂交鳢均比亲本小,差异显著(P<0.05);杂交鳢及其亲本血液中都含有较少比例的DNA合成期(S期)细胞,但彼此差异不显著(P>0.05)。     

赣昌鲫(日本白鲫♀×兴国红鲤♂)及其双亲同工酶的研究

采用不连续聚丙酰胺凝胶电泳法,研究了赣昌鲫(Carassius cuvieri♀×Cyprinus carpio var. singuonensis ♂)及其双亲的肌肉、肝脏、肾脏、心脏、脑、晶状体6种组织在超氧化物歧化酶(SOD)、苹果酸脱氢酶同工酶(MDH)、乳酸脱氢酶同工酶(LDH)、酯酶(EST)四种同工酶酶谱表达上的遗传差异。结果显示:赣昌鲫的SOD酶谱表达在6种组织中与母本一致;心脏、肝脏、肾脏的MDH表达酶带数与母本相同,且具偏母遗传特性,肌肉的MDH表达谱带数与父本肌肉相同,具偏父遗传特性;LDH、EST在各组织中的酶带表达形式则多数特征偏向于父本,部分与母本相似;LDH同工酶的表达显示出新杂种酶带特异性,EST同工酶在肾脏中也表达了自身特有的杂种酶带。结果表明,属间杂交的后代遗传物质上也存在着变异,存在可选育的性状。     

栽培基质及配方施肥对虎雪兰‘霞光’的生长影响试验

通过盆栽方式，采用不同体积混合配比的泥炭、椰糠、陶粒、树皮基质试验，以N、P、K3元素3水平设置了正交施肥试验，测定植株的生长状况、叶片养分及发病情况，分析了不同栽培基质和施肥处理对‘霞光’生长特性的影响。结果表明，混合栽培基质对虎雪兰‘霞光’的生长较单一的基质效果好，以1／4树皮4-1／4泥炭＋1／4椰糠＋1／4陶粒混合基质植株的株高、假鳞茎、叶面积和根系生长效果最佳；外施氮、磷、钾肥最佳组合为N1P2K3，即NH4N03、NaH2P04和KCl含量分别为1．25mmol／L、0．456mmol／L和2．62mmol／L，有利于虎雪兰‘霞光’植株的营养生长，且设施栽培中发病率较低。     

杂交马褂木幼林施肥试验

以杂交马褂木幼林为供试对象，进行不同肥料种类和施肥量试验。结果表明：追肥1a后，各施肥处理间树高生长量有极显著差异，以施洞氮复合肥0.25kg／株和马褂木无机专用肥0.25k∥株的树高生长量最大；追肥3a后，各施肥处理间树高生长量有极显著差异，胸径生长量没有显著差异，以施尿素0.25kg／株的树高生长量最大，胸径和树高生长量分别为3.09em和1.85m，比对照分别提高72％和111％。综合成本效益考虑，适合推广的肥料种类和施肥量依次为马褂木无机专用肥0.1ks／株、马褂木无机专用肥0.25k∥株和尿素0.25kg／株。     

不同施肥处理对湿加松早期生长及感病指数的影响

调查了福建省德化葛坑国有林场4种不同施肥处理后,2、3、4年生湿加松树高、胸（地）径和病害指数变化情况。结果表明,（1）不同施肥处理对湿加松早期树高、胸（地）径和松感病指数均产生显著影响,各生长年度树高、胸（地）径生长大小依次为：施氮磷钾复合肥与硼砂〉施氮磷肥与硼砂〉施氮磷肥〉对照（不施肥）,而感病指数大小则为：对照（...     

夏秋用双限性家蚕品种1303×1302的选育

选用限性基础材料,采用连续回交的育种方法,育成了夏秋用双限性家蚕品种1303、1302。新品种经高温多湿定向选育,抗逆性强,健康好养,万蚕产茧量18.39 kg,茧丝长1180.5 m、解舒丝长968.8 m、解舒率82.04%,鲜茧出丝率17.92%,洁净96.0分。新品种具有可利用幼虫斑纹区分雌雄的特性,可作为生产优质蚕种和雌雄分缫茧丝的专用蚕品种。2005年该品种通过四川省家蚕品种审定委员会审定。     

营养调控对母猪繁殖性能的影响

探讨妊娠母猪妊娠早期日粮中补加一定量的甲基吡啶铬、叶酸、维生素E和维生素A对母猪窝产仔数、初生重、仔猪成活率的影响。试验分为后备母猪和经产母猪两个组,并设相应的对照组,通过试验得出妊娠母猪妊娠早期日粮中补加一定量的甲基吡啶铬、叶酸、维生素E和维生素A,结果显著地提高母猪窝产仔数,平均为29.5%,对初生重和仔猪成活率无显著影响。在母猪妊娠早期的日粮中补加甲基吡啶铬和叶酸等进行营养调控,可提高母猪的繁殖性能。